diff --git a/.changeset/fix-test-api-pair-inference.md b/.changeset/fix-test-api-pair-inference.md
new file mode 100644
index 0000000000..d527a27600
--- /dev/null
+++ b/.changeset/fix-test-api-pair-inference.md
@@ -0,0 +1,5 @@
+---
+'@backstage/frontend-test-utils': patch
+---
+
+Fixed type inference of `TestApiPair` when using tuple syntax by wrapping `MockWithApiFactory` in `NoInfer`.
diff --git a/.patches/pr-33057.txt b/.patches/pr-33057.txt
new file mode 100644
index 0000000000..3499e84a89
--- /dev/null
+++ b/.patches/pr-33057.txt
@@ -0,0 +1 @@
+Fixed a type inference issue with the `apis` option of testing utilities in `@backstage/frontend-test-utils`.
\ No newline at end of file
diff --git a/packages/frontend-test-utils/report.api.md b/packages/frontend-test-utils/report.api.md
index 1cba02f393..a17c2ed483 100644
--- a/packages/frontend-test-utils/report.api.md
+++ b/packages/frontend-test-utils/report.api.md
@@ -443,7 +443,7 @@ export type RenderTestAppOptions<TApiPairs extends any[] = any[]> = {
 // @public
 export type TestApiPair<TApi> =
   | readonly [ApiRef<TApi>, TApi extends infer TImpl ? Partial<TImpl> : never]
-  | MockWithApiFactory<TApi>;
+  | MockWithApiFactory<NoInfer<TApi>>;
 
 // @public
 export type TestApiPairs<TApiPairs> = {
diff --git a/packages/frontend-test-utils/src/apis/TestApiProvider.test.tsx b/packages/frontend-test-utils/src/apis/TestApiProvider.test.tsx
new file mode 100644
index 0000000000..1bb5673751
--- /dev/null
+++ b/packages/frontend-test-utils/src/apis/TestApiProvider.test.tsx
@@ -0,0 +1,95 @@
+/*
+ * Copyright 2020 The Backstage Authors
+ *
+ * Licensed under the Apache License, Version 2.0 (the "License");
+ * you may not use this file except in compliance with the License.
+ * You may obtain a copy of the License at
+ *
+ *     http://www.apache.org/licenses/LICENSE-2.0
+ *
+ * Unless required by applicable law or agreed to in writing, software
+ * distributed under the License is distributed on an "AS IS" BASIS,
+ * WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+ * See the License for the specific language governing permissions and
+ * limitations under the License.
+ */
+
+import { createApiRef } from '@backstage/frontend-plugin-api';
+import { TestApiProvider } from './TestApiProvider';
+import { mockApis } from './mockApis';
+import { render, screen } from '@testing-library/react';
+
+const xApiRef = createApiRef<{ a: string; b: number }>({
+  id: 'x',
+});
+const yApiRef = createApiRef<string>({
+  id: 'y',
+});
+
+describe('TestApiProvider', () => {
+  it('should provide tuple APIs and check types', () => {
+    render(
+      <TestApiProvider
+        apis={[
+          [xApiRef, { a: 'a', b: 3 }],
+          [yApiRef, 'y'],
+        ]}
+      >
+        <div />
+      </TestApiProvider>,
+    );
+  });
+
+  it('should allow partial API implementations', () => {
+    render(
+      <TestApiProvider apis={[[xApiRef, { a: 'a' }]]}>
+        <div />
+      </TestApiProvider>,
+    );
+  });
+
+  it('should reject mismatched types in tuple syntax', () => {
+    render(
+      // @ts-expect-error - a should be a string, not a number
+      <TestApiProvider apis={[[xApiRef, { a: 3 }]]}>
+        <div />
+      </TestApiProvider>,
+    );
+  });
+
+  it('should accept MockWithApiFactory entries', () => {
+    render(
+      <TestApiProvider apis={[mockApis.alert()]}>
+        <div />
+      </TestApiProvider>,
+    );
+  });
+
+  it('should accept a mix of tuples and MockWithApiFactory entries', () => {
+    render(
+      <TestApiProvider apis={[[xApiRef, { a: 'a' }], mockApis.alert()]}>
+        <div />
+      </TestApiProvider>,
+    );
+  });
+
+  it('should allow empty APIs', () => {
+    render(
+      <TestApiProvider apis={[]}>
+        <div />
+      </TestApiProvider>,
+    );
+  });
+
+  it('should provide APIs at runtime', async () => {
+    const alertApi = mockApis.alert();
+
+    render(
+      <TestApiProvider apis={[[xApiRef, { a: 'hello', b: 42 }], alertApi]}>
+        <span>rendered</span>
+      </TestApiProvider>,
+    );
+
+    expect(await screen.findByText('rendered')).toBeInTheDocument();
+  });
+});
diff --git a/packages/frontend-test-utils/src/apis/TestApiProvider.tsx b/packages/frontend-test-utils/src/apis/TestApiProvider.tsx
index 215e8c8a71..d0eca87a33 100644
--- a/packages/frontend-test-utils/src/apis/TestApiProvider.tsx
+++ b/packages/frontend-test-utils/src/apis/TestApiProvider.tsx
@@ -29,7 +29,7 @@ import {
  */
 export type TestApiPair<TApi> =
   | readonly [ApiRef<TApi>, TApi extends infer TImpl ? Partial<TImpl> : never]
-  | MockWithApiFactory<TApi>;
+  | MockWithApiFactory<NoInfer<TApi>>;
 
 /**
  * Represents an array of mock API implementation.