diff --git a/.changeset/happy-cows-moo.md b/.changeset/happy-cows-moo.md
new file mode 100644
index 0000000000..a5e425f5d2
--- /dev/null
+++ b/.changeset/happy-cows-moo.md
@@ -0,0 +1,5 @@
+---
+'@backstage/plugin-azure-devops-backend': patch
+---
+
+Fixed a bug where the azure devops host in URLs on the readme card was being URL encoded, breaking hosts with ports.
diff --git a/plugins/azure-devops-backend/src/utils/azure-devops-utils.test.ts b/plugins/azure-devops-backend/src/utils/azure-devops-utils.test.ts
index 4b912a8340..427980be14 100644
--- a/plugins/azure-devops-backend/src/utils/azure-devops-utils.test.ts
+++ b/plugins/azure-devops-backend/src/utils/azure-devops-utils.test.ts
@@ -27,6 +27,7 @@ import {
   getAvatarUrl,
   getPullRequestLink,
   replaceReadme,
+  buildEncodedUrl,
 } from './azure-devops-utils';
 import { GitPullRequest } from 'azure-devops-node-api/interfaces/GitInterfaces';
 import { UrlReader } from '@backstage/backend-common';
@@ -278,3 +279,19 @@ describe('replaceReadme', () => {
     expect(expected).toBe(result);
   });
 });
+
+describe('buildEncodedUrl', () => {
+  it('should not encode the colon between host and port', async () => {
+    const result = await buildEncodedUrl(
+      'tfs.myorg.com:8443',
+      'org',
+      'project',
+      'repo',
+      'path',
+    );
+
+    expect(result).toBe(
+      'https://tfs.myorg.com:8443/org/project/_git/repo?path=path',
+    );
+  });
+});
diff --git a/plugins/azure-devops-backend/src/utils/azure-devops-utils.ts b/plugins/azure-devops-backend/src/utils/azure-devops-utils.ts
index 0aafb54a0a..aa946e7a74 100644
--- a/plugins/azure-devops-backend/src/utils/azure-devops-utils.ts
+++ b/plugins/azure-devops-backend/src/utils/azure-devops-utils.ts
@@ -242,12 +242,11 @@ export function buildEncodedUrl(
   repo: string,
   path: string,
 ): string {
-  const encodedHost = encodeURIComponent(host);
   const encodedOrg = encodeURIComponent(org);
   const encodedProject = encodeURIComponent(project);
   const encodedRepo = encodeURIComponent(repo);
   const encodedPath = encodeURIComponent(path);
-  return `https://${encodedHost}/${encodedOrg}/${encodedProject}/_git/${encodedRepo}?path=${encodedPath}`;
+  return `https://${host}/${encodedOrg}/${encodedProject}/_git/${encodedRepo}?path=${encodedPath}`;
 }
 
 function convertReviewer(