diff --git a/.changeset/quick-states-enjoy.md b/.changeset/quick-states-enjoy.md
new file mode 100644
index 0000000000..b6c5303c48
--- /dev/null
+++ b/.changeset/quick-states-enjoy.md
@@ -0,0 +1,5 @@
+---
+'@backstage/plugin-user-settings-backend': patch
+---
+
+Resolves an issue where user setting keys containing slashes returned 404 not found.
diff --git a/plugins/user-settings-backend/src/service/router.test.ts b/plugins/user-settings-backend/src/service/router.test.ts
index afc725025c..01277a9fd3 100644
--- a/plugins/user-settings-backend/src/service/router.test.ts
+++ b/plugins/user-settings-backend/src/service/router.test.ts
@@ -74,6 +74,30 @@ describe('createRouter', () => {
       });
     });
 
+    it('returns ok for keys with forward slashes', async () => {
+      const setting = {
+        bucket: 'my-bucket',
+        key: 'my-key/with/slashes',
+        value: 'a',
+      };
+
+      userSettingsStore.get.mockResolvedValue(setting);
+
+      const responses = await request(app).get(
+        '/buckets/my-bucket/keys/my-key/with/slashes',
+      );
+
+      expect(responses.status).toEqual(200);
+      expect(responses.body).toEqual(setting);
+
+      expect(userSettingsStore.get).toHaveBeenCalledTimes(1);
+      expect(userSettingsStore.get).toHaveBeenCalledWith({
+        userEntityRef: mockUserRef,
+        bucket: 'my-bucket',
+        key: 'my-key/with/slashes',
+      });
+    });
+
     it('returns an error if the Authorization header is missing', async () => {
       const responses = await request(app)
         .get('/buckets/my-bucket/keys/my-key')
@@ -107,6 +131,28 @@ describe('createRouter', () => {
       });
     });
 
+    it('returns ok for keys with forward slashes', async () => {
+      userSettingsStore.delete.mockResolvedValue();
+
+      const responses = await request(app).delete(
+        '/buckets/my-bucket/keys/my-key/with/slashes',
+      );
+
+      expect(responses.status).toEqual(204);
+
+      expect(userSettingsStore.delete).toHaveBeenCalledTimes(1);
+      expect(userSettingsStore.delete).toHaveBeenCalledWith({
+        userEntityRef: mockUserRef,
+        bucket: 'my-bucket',
+        key: 'my-key/with/slashes',
+      });
+      expect(signalService.publish).toHaveBeenCalledWith({
+        recipients: { type: 'user', entityRef: mockUserRef },
+        channel: `user-settings`,
+        message: { type: 'key-deleted', key: 'my-key/with/slashes' },
+      });
+    });
+
     it('returns an error if the Authorization header is missing', async () => {
       const responses = await request(app)
         .delete('/buckets/my-bucket/keys/my-key')
@@ -151,6 +197,26 @@ describe('createRouter', () => {
       });
     });
 
+    it('returns ok for keys with forward slashes', async () => {
+      const setting = {
+        bucket: 'my-bucket',
+        key: 'my-key/with/slashes',
+        value: 'a',
+      };
+
+      userSettingsStore.set.mockResolvedValue();
+      userSettingsStore.get.mockResolvedValue(setting);
+
+      const responses = await request(app)
+        .put('/buckets/my-bucket/keys/my-key/with/slashes')
+        .send({ value: 'a' });
+
+      expect(responses.status).toEqual(200);
+      expect(responses.body).toEqual(setting);
+
+      expect(userSettingsStore.set).toHaveBeenCalledTimes(1);
+    });
+
     it('returns an error if the value is not given', async () => {
       const responses = await request(app)
         .put('/buckets/my-bucket/keys/my-key')
diff --git a/plugins/user-settings-backend/src/service/router.ts b/plugins/user-settings-backend/src/service/router.ts
index 07b4443551..3a0de7054c 100644
--- a/plugins/user-settings-backend/src/service/router.ts
+++ b/plugins/user-settings-backend/src/service/router.ts
@@ -41,7 +41,7 @@ export async function createRouter(options: {
   };
 
   // get a single value
-  router.get('/buckets/:bucket/keys/:key', async (req, res) => {
+  router.get('/buckets/:bucket/keys/:key(*)', async (req, res) => {
     const userEntityRef = await getUserEntityRef(req);
     const { bucket, key } = req.params;
 
@@ -55,7 +55,7 @@ export async function createRouter(options: {
   });
 
   // set a single value
-  router.put('/buckets/:bucket/keys/:key', async (req, res) => {
+  router.put('/buckets/:bucket/keys/:key(*)', async (req, res) => {
     const userEntityRef = await getUserEntityRef(req);
     const { bucket, key } = req.params;
     const { value } = req.body;
@@ -87,8 +87,8 @@ export async function createRouter(options: {
     res.json(setting);
   });
 
-  // get a single value
-  router.delete('/buckets/:bucket/keys/:key', async (req, res) => {
+  // delete a single value
+  router.delete('/buckets/:bucket/keys/:key(*)', async (req, res) => {
     const userEntityRef = await getUserEntityRef(req);
     const { bucket, key } = req.params;