diff --git a/.changeset/strong-sloths-push.md b/.changeset/strong-sloths-push.md
new file mode 100644
index 0000000000..5e449b21ef
--- /dev/null
+++ b/.changeset/strong-sloths-push.md
@@ -0,0 +1,5 @@
+---
+'@backstage/plugin-scaffolder-backend': minor
+---
+
+Allow using globby's negative matching with copyWithoutTemplating/copyWithoutRender. Allows including an entire subdirectory while excluding a single file so that it will still be templated instead of needing to list every other file and ensure the list is updated when new files are added.
diff --git a/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.test.ts b/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.test.ts
index d396a00c26..119cd78eb7 100644
--- a/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.test.ts
+++ b/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.test.ts
@@ -470,6 +470,57 @@ describe('fetch:template', () => {
         ),
       ).resolves.toEqual('1234');
     });
+
+    describe('with exclusion filter', () => {
+      beforeEach(async () => {
+        context = mockContext({
+          values: {
+            name: 'test-project',
+            count: 1234,
+          },
+          copyWithoutTemplating: [
+            '.unprocessed',
+            '!*/templated-process-content-${{ values.name }}.txt',
+          ],
+        });
+
+        mockFetchContents.mockImplementation(({ outputPath }) => {
+          mockDir.setContent({
+            [outputPath]: {
+              processed: {
+                'templated-content-${{ values.name }}.txt':
+                  '${{ values.count }}',
+              },
+              '.unprocessed': {
+                'templated-content-${{ values.name }}.txt':
+                  '${{ values.count }}',
+                'templated-process-content-${{ values.name }}.txt':
+                  '${{ values.count }}',
+              },
+            },
+          });
+
+          return Promise.resolve();
+        });
+
+        await action.handler(context);
+      });
+
+      it('renders path template including excluded matches in copyWithoutTemplating', async () => {
+        await expect(
+          fs.readFile(
+            `${workspacePath}/target/.unprocessed/templated-process-content-test-project.txt`,
+            'utf-8',
+          ),
+        ).resolves.toEqual('1234');
+        await expect(
+          fs.readFile(
+            `${workspacePath}/target/.unprocessed/templated-content-test-project.txt`,
+            'utf-8',
+          ),
+        ).resolves.toEqual('${{ values.count }}');
+      });
+    });
   });
 
   describe('cookiecutter compatibility mode', () => {
diff --git a/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.ts b/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.ts
index 1116d4dd5d..26b249b76d 100644
--- a/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.ts
+++ b/plugins/scaffolder-backend/src/scaffolder/actions/builtin/fetch/template.ts
@@ -207,19 +207,13 @@ export function createFetchTemplateAction(options: {
       });
 
       const nonTemplatedEntries = new Set(
-        (
-          await Promise.all(
-            (copyOnlyPatterns || []).map(pattern =>
-              globby(pattern, {
-                cwd: templateDir,
-                dot: true,
-                onlyFiles: false,
-                markDirectories: true,
-                followSymbolicLinks: false,
-              }),
-            ),
-          )
-        ).flat(),
+        await globby(copyOnlyPatterns || [], {
+          cwd: templateDir,
+          dot: true,
+          onlyFiles: false,
+          markDirectories: true,
+          followSymbolicLinks: false,
+        }),
       );
 
       // Cookiecutter prefixes all parameters in templates with