diff --git a/.changeset/yellow-schools-relax.md b/.changeset/yellow-schools-relax.md
new file mode 100644
index 0000000000..fd5f1fa7f7
--- /dev/null
+++ b/.changeset/yellow-schools-relax.md
@@ -0,0 +1,5 @@
+---
+'@backstage/theme': patch
+---
+
+Override the spacing to a v5 compliant method
diff --git a/packages/theme/src/unified/overrides.test.ts b/packages/theme/src/unified/overrides.test.ts
index e84cedade6..c99c0c10a4 100644
--- a/packages/theme/src/unified/overrides.test.ts
+++ b/packages/theme/src/unified/overrides.test.ts
@@ -18,12 +18,14 @@ import { Theme } from '@mui/material/styles';
 import { transformV5ComponentThemesToV4 } from './overrides';
 
 describe('transformV5ComponentThemesToV4', () => {
+  // TODO: Create actual v5 Theme to avoid missing properties in test
   const mockTheme = {
     palette: {
       primary: {
         main: 'red',
       },
     },
+    spacing: (n: number) => `${n * 8}px`,
   } as unknown as Theme;
   it('transforms empty component themes', () => {
     expect(transformV5ComponentThemesToV4(mockTheme)).toEqual({
diff --git a/packages/theme/src/unified/overrides.ts b/packages/theme/src/unified/overrides.ts
index 7417fccedc..a87f047f5c 100644
--- a/packages/theme/src/unified/overrides.ts
+++ b/packages/theme/src/unified/overrides.ts
@@ -29,6 +29,26 @@ type StaticStyleRules = Record<
   CSSProperties | Record<string, CSSProperties>
 >;
 
+// Utility function based on v5 `createSpacing`: https://github.com/mui/material-ui/blob/master/packages/mui-system/src/createTheme/createSpacing.ts#L42C3-L59C5
+const __v5Spacing =
+  (defaultSpacing: number) =>
+  (...argsInput: ReadonlyArray<number | string>) => {
+    const args = argsInput.length === 0 ? [1] : argsInput;
+    const transform = (argument: string | number, themeSpacing: number) => {
+      if (typeof argument === 'string') {
+        return argument;
+      }
+      return themeSpacing * argument;
+    };
+
+    return args
+      .map(argument => {
+        const output = transform(argument, defaultSpacing);
+        return typeof output === 'number' ? `${output}px` : output;
+      })
+      .join(' ');
+  };
+
 // Converts callback-based overrides to static styles, e.g.
 // { root: theme => ({ color: theme.color }) } -> { root: { color: 'red' } }
 function adaptV5CssBaselineOverride(
@@ -60,6 +80,12 @@ function adaptV5Override(
     return Object.fromEntries(
       Object.entries(overrides).map(([className, style]) => {
         if (typeof style === 'function') {
+          const defaultSpacing = theme.spacing(1);
+          if (typeof defaultSpacing === 'number') {
+            // Override potential v4 spacing method: https://mui.com/material-ui/migration/v5-style-changes/#%E2%9C%85-remove-px-suffix
+            // `adaptV4Theme as reference: https://github.com/mui/material-ui/blob/v5.x/packages/mui-material/src/styles/adaptV4Theme.js#L54C41-L54C41
+            theme.spacing = __v5Spacing(defaultSpacing);
+          }
           return [className, style({ theme })];
         }
         return [className, style];