diff --git a/.changeset/itchy-news-drive.md b/.changeset/itchy-news-drive.md
new file mode 100644
index 0000000000..97cca5b155
--- /dev/null
+++ b/.changeset/itchy-news-drive.md
@@ -0,0 +1,15 @@
+---
+'@backstage/plugin-azure-devops-backend': minor
+'@backstage/plugin-azure-devops-common': minor
+'@backstage/plugin-azure-devops': minor
+---
+
+Ability to fetch the README file from a different Azure DevOps path.
+
+Defaults to the current, Azure DevOps default behaviour (`README.md` in the root of the git repo); to use a different path, add the annotation `dev.azure.com/readme-path`
+
+Example:
+
+```yaml
+dev.azure.com/readme-path: /my-path/README.md
+```
diff --git a/plugins/azure-devops-backend/api-report.md b/plugins/azure-devops-backend/api-report.md
index 6dead7f11b..d98d178cff 100644
--- a/plugins/azure-devops-backend/api-report.md
+++ b/plugins/azure-devops-backend/api-report.md
@@ -124,6 +124,7 @@ export class AzureDevOpsApi {
     org: string,
     project: string,
     repo: string,
+    path: string,
   ): Promise<{
     url: string;
     content: string;
diff --git a/plugins/azure-devops-backend/package.json b/plugins/azure-devops-backend/package.json
index 37a39e6ffe..2d5300a62a 100644
--- a/plugins/azure-devops-backend/package.json
+++ b/plugins/azure-devops-backend/package.json
@@ -32,6 +32,7 @@
     "@backstage/backend-plugin-api": "workspace:^",
     "@backstage/catalog-model": "workspace:^",
     "@backstage/config": "workspace:^",
+    "@backstage/errors": "workspace:^",
     "@backstage/integration": "workspace:^",
     "@backstage/plugin-azure-devops-common": "workspace:^",
     "@backstage/plugin-catalog-common": "workspace:^",
diff --git a/plugins/azure-devops-backend/src/api/AzureDevOpsApi.ts b/plugins/azure-devops-backend/src/api/AzureDevOpsApi.ts
index d50888006b..cc5cad92b7 100644
--- a/plugins/azure-devops-backend/src/api/AzureDevOpsApi.ts
+++ b/plugins/azure-devops-backend/src/api/AzureDevOpsApi.ts
@@ -527,11 +527,12 @@ export class AzureDevOpsApi {
     org: string,
     project: string,
     repo: string,
+    path: string,
   ): Promise<{
     url: string;
     content: string;
   }> {
-    const url = buildEncodedUrl(host, org, project, repo, 'README.md');
+    const url = buildEncodedUrl(host, org, project, repo, path);
     const response = await this.urlReader.readUrl(url);
     const buffer = await response.buffer();
     const content = await replaceReadme(
diff --git a/plugins/azure-devops-backend/src/service/router.test.ts b/plugins/azure-devops-backend/src/service/router.test.ts
index 65cb27271e..c12b13a5aa 100644
--- a/plugins/azure-devops-backend/src/service/router.test.ts
+++ b/plugins/azure-devops-backend/src/service/router.test.ts
@@ -482,7 +482,7 @@ describe('createRouter', () => {
   });
 
   describe('GET /readme/:projectName/:repoName', () => {
-    it('fetches readme file', async () => {
+    it('fetches default default readme file', async () => {
       const content = getReadmeMock();
       const url = `https://host.com/myOrg/myProject/_git/myRepo?path=README.md`;
 
@@ -491,14 +491,13 @@ describe('createRouter', () => {
         url,
       });
 
-      const response = await request(app).get(
-        '/readme/myProject/myRepo?path=README.md',
-      );
+      const response = await request(app).get('/readme/myProject/myRepo');
       expect(azureDevOpsApi.getReadme).toHaveBeenCalledWith(
         'host.com',
         'myOrg',
         'myProject',
         'myRepo',
+        'README.md',
       );
       expect(response.status).toEqual(200);
       expect(response.body).toEqual({
@@ -507,6 +506,80 @@ describe('createRouter', () => {
       });
     });
   });
+
+  describe('GET /readme/:projectName/:repoName with readme filename', () => {
+    it('fetches specified readme file', async () => {
+      const content = getReadmeMock();
+      const url = `https://host.com/myOrg/myProject/_git/myRepo?path=README_NOT_DEFAULT.md`;
+
+      azureDevOpsApi.getReadme.mockResolvedValueOnce({
+        content,
+        url,
+      });
+
+      const response = await request(app).get(
+        '/readme/myProject/myRepo?path=README_NOT_DEFAULT.md',
+      );
+      expect(azureDevOpsApi.getReadme).toHaveBeenCalledWith(
+        'host.com',
+        'myOrg',
+        'myProject',
+        'myRepo',
+        'README_NOT_DEFAULT.md',
+      );
+      expect(response.status).toEqual(200);
+      expect(response.body).toEqual({
+        content,
+        url,
+      });
+    });
+  });
+
+  describe('GET /readme/:projectName/:repoName with readme path', () => {
+    it('fetches specified readme file from subfolder', async () => {
+      const content = getReadmeMock();
+      const url = `https://host.com/myOrg/myProject/_git/myRepo?path=/my-path/README.md`;
+
+      azureDevOpsApi.getReadme.mockResolvedValueOnce({
+        content,
+        url,
+      });
+
+      const response = await request(app).get(
+        '/readme/myProject/myRepo?path=/my-path/README.md',
+      );
+      expect(azureDevOpsApi.getReadme).toHaveBeenCalledWith(
+        'host.com',
+        'myOrg',
+        'myProject',
+        'myRepo',
+        '/my-path/README.md',
+      );
+      expect(response.status).toEqual(200);
+      expect(response.body).toEqual({
+        content,
+        url,
+      });
+    });
+  });
+
+  describe('GET /readme/:projectName/:repoName with a bad readme path (multiple values)', () => {
+    it('throws InputError', async () => {
+      const response = await request(app).get(
+        '/readme/myProject/myRepo?path=1&path=2',
+      );
+      expect(azureDevOpsApi.getReadme).not.toHaveBeenCalled();
+      expect(response.status).toEqual(400);
+    });
+  });
+
+  describe('GET /readme/:projectName/:repoName with a bad readme path (empty string)', () => {
+    it('throws InputError', async () => {
+      const response = await request(app).get('/readme/myProject/myRepo?path=');
+      expect(azureDevOpsApi.getReadme).not.toHaveBeenCalled();
+      expect(response.status).toEqual(400);
+    });
+  });
 });
 
 function getReadmeMock() {
diff --git a/plugins/azure-devops-backend/src/service/router.ts b/plugins/azure-devops-backend/src/service/router.ts
index 9a71c8f03d..bc13a4eee2 100644
--- a/plugins/azure-devops-backend/src/service/router.ts
+++ b/plugins/azure-devops-backend/src/service/router.ts
@@ -26,6 +26,7 @@ import { Logger } from 'winston';
 import { PullRequestsDashboardProvider } from '../api/PullRequestsDashboardProvider';
 import Router from 'express-promise-router';
 import { errorHandler, UrlReader } from '@backstage/backend-common';
+import { InputError } from '@backstage/errors';
 import express from 'express';
 
 const DEFAULT_TOP = 10;
@@ -216,12 +217,28 @@ export async function createRouter(
       req.query.host?.toString() ?? config.getString('azureDevOps.host');
     const org =
       req.query.org?.toString() ?? config.getString('azureDevOps.organization');
+    let path = req.query.path;
+
+    if (path === undefined) {
+      // if the annotation is missing, default to the previous behaviour (look for README.md in the root of the repo)
+      path = 'README.md';
+    }
+
+    if (typeof path !== 'string') {
+      throw new InputError('Invalid path param');
+    }
+
+    if (path === '') {
+      throw new InputError('If present, the path param should not be empty');
+    }
+
     const { projectName, repoName } = req.params;
     const readme = await azureDevOpsApi.getReadme(
       host,
       org,
       projectName,
       repoName,
+      path,
     );
     res.status(200).json(readme);
   });
diff --git a/plugins/azure-devops-common/api-report.md b/plugins/azure-devops-common/api-report.md
index 5bd1674fc8..e8343178e3 100644
--- a/plugins/azure-devops-common/api-report.md
+++ b/plugins/azure-devops-common/api-report.md
@@ -16,6 +16,9 @@ export const AZURE_DEVOPS_HOST_ORG_ANNOTATION = 'dev.azure.com/host-org';
 // @public (undocumented)
 export const AZURE_DEVOPS_PROJECT_ANNOTATION = 'dev.azure.com/project';
 
+// @public (undocumented)
+export const AZURE_DEVOPS_README_ANNOTATION = 'dev.azure.com/readme-path';
+
 // @public (undocumented)
 export const AZURE_DEVOPS_REPO_ANNOTATION = 'dev.azure.com/project-repo';
 
@@ -228,6 +231,8 @@ export interface ReadmeConfig {
   // (undocumented)
   org?: string;
   // (undocumented)
+  path?: string;
+  // (undocumented)
   project: string;
   // (undocumented)
   repo: string;
diff --git a/plugins/azure-devops-common/src/constants.ts b/plugins/azure-devops-common/src/constants.ts
index 5cc6b54592..05eb430cc9 100644
--- a/plugins/azure-devops-common/src/constants.ts
+++ b/plugins/azure-devops-common/src/constants.ts
@@ -22,6 +22,8 @@ export const AZURE_DEVOPS_HOST_ORG_ANNOTATION = 'dev.azure.com/host-org';
 /** @public */
 export const AZURE_DEVOPS_PROJECT_ANNOTATION = 'dev.azure.com/project';
 /** @public */
+export const AZURE_DEVOPS_README_ANNOTATION = 'dev.azure.com/readme-path';
+/** @public */
 export const AZURE_DEVOPS_REPO_ANNOTATION = 'dev.azure.com/project-repo';
 /** @public */
 export const AZURE_DEVOPS_DEFAULT_TOP: number = 10;
diff --git a/plugins/azure-devops-common/src/types.ts b/plugins/azure-devops-common/src/types.ts
index afb6083867..0390bb1f6e 100644
--- a/plugins/azure-devops-common/src/types.ts
+++ b/plugins/azure-devops-common/src/types.ts
@@ -212,6 +212,7 @@ export interface ReadmeConfig {
   repo: string;
   host?: string;
   org?: string;
+  path?: string;
 }
 
 /** @public */
diff --git a/plugins/azure-devops/README.md b/plugins/azure-devops/README.md
index f897588987..935e78c82c 100644
--- a/plugins/azure-devops/README.md
+++ b/plugins/azure-devops/README.md
@@ -63,13 +63,19 @@ spec:
 
 #### Mono repos
 
-If you have multiple entities within a single repo, you will need to specify which pipelines belong to each entity.
+If you have multiple entities within a single repo, you will need to specify which pipelines belong to each entity, like this:
 
 ```yaml
 dev.azure.com/project-repo: <my-project>/<my-repo>
 dev.azure.com/build-definition: <build-definition-name>
 ```
 
+Then to display the `README` file that belongs to each entity you would do this:
+
+```yaml
+dev.azure.com/readme-path: /<path-to>/<my-readme-file>.md
+```
+
 #### Pipeline in different project to repo
 
 If your pipeline is in a different project to the source code, you will need to specify this in the project annotation.
diff --git a/plugins/azure-devops/src/api/AzureDevOpsClient.ts b/plugins/azure-devops/src/api/AzureDevOpsClient.ts
index 76e38b064c..a10b10ff14 100644
--- a/plugins/azure-devops/src/api/AzureDevOpsClient.ts
+++ b/plugins/azure-devops/src/api/AzureDevOpsClient.ts
@@ -189,6 +189,9 @@ export class AzureDevOpsClient implements AzureDevOpsApi {
     if (opts.org) {
       queryString.append('org', opts.org);
     }
+    if (opts.path) {
+      queryString.append('path', opts.path);
+    }
     return await this.get(
       `readme/${encodeURIComponent(opts.project)}/${encodeURIComponent(
         opts.repo,
diff --git a/plugins/azure-devops/src/hooks/useReadme.ts b/plugins/azure-devops/src/hooks/useReadme.ts
index 348b4219f0..9aa376e0bd 100644
--- a/plugins/azure-devops/src/hooks/useReadme.ts
+++ b/plugins/azure-devops/src/hooks/useReadme.ts
@@ -30,8 +30,15 @@ export function useReadme(entity: Entity): {
   const api = useApi(azureDevOpsApiRef);
 
   const { value, loading, error } = useAsync(() => {
-    const { project, repo, host, org } = getAnnotationValuesFromEntity(entity);
-    return api.getReadme({ project, repo: repo as string, host, org });
+    const { project, repo, host, org, readmePath } =
+      getAnnotationValuesFromEntity(entity);
+    return api.getReadme({
+      project,
+      repo: repo as string,
+      host,
+      org,
+      path: readmePath,
+    });
   }, [api]);
 
   return {
diff --git a/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.test.ts b/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.test.ts
index 9325a79039..e196e9dfc7 100644
--- a/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.test.ts
+++ b/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.test.ts
@@ -52,6 +52,7 @@ describe('getAnnotationValuesFromEntity', () => {
         project: 'projectName',
         repo: 'repoName',
         definition: undefined,
+        readmePath: undefined,
         host: undefined,
         org: undefined,
       });
@@ -149,6 +150,7 @@ describe('getAnnotationValuesFromEntity', () => {
         project: 'projectName',
         repo: undefined,
         definition: 'buildDefinitionName',
+        readmePath: undefined,
         host: undefined,
         org: undefined,
       });
@@ -220,6 +222,7 @@ describe('getAnnotationValuesFromEntity', () => {
         project: 'projectName',
         repo: 'repoName',
         definition: undefined,
+        readmePath: undefined,
         host: 'hostName',
         org: 'organizationName',
       });
@@ -246,6 +249,7 @@ describe('getAnnotationValuesFromEntity', () => {
         project: 'projectName',
         repo: undefined,
         definition: 'buildDefinitionName',
+        readmePath: undefined,
         host: 'hostName',
         org: 'organizationName',
       });
@@ -344,6 +348,7 @@ describe('getAnnotationValuesFromEntity', () => {
         project: 'projectName',
         repo: 'repoName',
         definition: undefined,
+        readmePath: undefined,
         host: 'company.com/tfs',
         org: 'organizationName',
       });
@@ -417,6 +422,7 @@ describe('getAnnotationValuesFromEntity', () => {
         project: 'projectName',
         repo: 'repoName',
         definition: 'buildDefinitionName',
+        readmePath: undefined,
         host: undefined,
         org: undefined,
       });
@@ -443,6 +449,87 @@ describe('getAnnotationValuesFromEntity', () => {
         project: 'projectName',
         repo: undefined,
         definition: 'buildDefinitionName',
+        readmePath: undefined,
+        host: undefined,
+        org: undefined,
+      });
+    });
+  });
+
+  describe('definition, project and readme', () => {
+    it('returns with the readme path', () => {
+      const entity: Entity = {
+        apiVersion: 'backstage.io/v1alpha1',
+        kind: 'Component',
+        metadata: {
+          namespace: 'default',
+          name: 'project-repo',
+          annotations: {
+            'dev.azure.com/project': 'projectName',
+            'dev.azure.com/build-definition': 'buildDefinitionName',
+            'dev.azure.com/readme-path': 'readme/path.md',
+          },
+        },
+      };
+      const values = getAnnotationValuesFromEntity(entity);
+      expect(values).toEqual({
+        project: 'projectName',
+        repo: undefined,
+        definition: 'buildDefinitionName',
+        readmePath: 'readme/path.md',
+        host: undefined,
+        org: undefined,
+      });
+    });
+  });
+
+  describe('definition, projectRepo and readme', () => {
+    it('returns with the readme path', () => {
+      const entity: Entity = {
+        apiVersion: 'backstage.io/v1alpha1',
+        kind: 'Component',
+        metadata: {
+          namespace: 'default',
+          name: 'project-repo',
+          annotations: {
+            'dev.azure.com/project-repo': 'projectName/repoName',
+            'dev.azure.com/build-definition': 'buildDefinitionName',
+            'dev.azure.com/readme-path': 'readme/path.md',
+          },
+        },
+      };
+      const values = getAnnotationValuesFromEntity(entity);
+      expect(values).toEqual({
+        project: 'projectName',
+        repo: 'repoName',
+        definition: 'buildDefinitionName',
+        readmePath: 'readme/path.md',
+        host: undefined,
+        org: undefined,
+      });
+    });
+  });
+
+  describe('projectRepo and readme', () => {
+    it('returns with the readme path', () => {
+      const entity: Entity = {
+        apiVersion: 'backstage.io/v1alpha1',
+        kind: 'Component',
+        metadata: {
+          namespace: 'default',
+          name: 'project-repo',
+          annotations: {
+            'dev.azure.com/project-repo': 'projectName/repoName',
+            'dev.azure.com/readme-path': 'readme/path.md',
+          },
+        },
+      };
+      const values = getAnnotationValuesFromEntity(entity);
+      expect(values).toEqual({
+        project: 'projectName',
+        repo: 'repoName',
+        definition: undefined,
+        readmePath: 'readme/path.md',
         host: undefined,
         org: undefined,
       });
diff --git a/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.ts b/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.ts
index 7533c473ed..fb38a531e1 100644
--- a/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.ts
+++ b/plugins/azure-devops/src/utils/getAnnotationValuesFromEntity.ts
@@ -18,6 +18,7 @@ import { Entity } from '@backstage/catalog-model';
 import {
   AZURE_DEVOPS_PROJECT_ANNOTATION,
   AZURE_DEVOPS_BUILD_DEFINITION_ANNOTATION,
+  AZURE_DEVOPS_README_ANNOTATION,
   AZURE_DEVOPS_REPO_ANNOTATION,
   AZURE_DEVOPS_HOST_ORG_ANNOTATION,
 } from '@backstage/plugin-azure-devops-common';
@@ -28,6 +29,7 @@ export function getAnnotationValuesFromEntity(entity: Entity): {
   definition?: string;
   host?: string;
   org?: string;
+  readmePath?: string;
 } {
   const hostOrg = getHostOrg(entity.metadata.annotations);
   const projectRepo = getProjectRepo(entity.metadata.annotations);
@@ -35,12 +37,15 @@ export function getAnnotationValuesFromEntity(entity: Entity): {
     entity.metadata.annotations?.[AZURE_DEVOPS_PROJECT_ANNOTATION];
   const definition =
     entity.metadata.annotations?.[AZURE_DEVOPS_BUILD_DEFINITION_ANNOTATION];
+  const readmePath =
+    entity.metadata.annotations?.[AZURE_DEVOPS_README_ANNOTATION];
 
   if (definition) {
     if (project) {
       return {
         project,
         definition,
+        readmePath: readmePath,
         ...hostOrg,
       };
     }
@@ -49,6 +54,7 @@ export function getAnnotationValuesFromEntity(entity: Entity): {
         project: projectRepo.project,
         repo: projectRepo.repo,
         definition,
+        readmePath: readmePath,
         ...hostOrg,
       };
     }
@@ -60,6 +66,7 @@ export function getAnnotationValuesFromEntity(entity: Entity): {
       return {
         project: projectRepo.project,
         repo: projectRepo.repo,
+        readmePath: readmePath,
         ...hostOrg,
       };
     }
diff --git a/yarn.lock b/yarn.lock
index 0a1a835df1..45de7b4d90 100644
--- a/yarn.lock
+++ b/yarn.lock
@@ -4933,6 +4933,7 @@ __metadata:
     "@backstage/catalog-model": "workspace:^"
     "@backstage/cli": "workspace:^"
     "@backstage/config": "workspace:^"
+    "@backstage/errors": "workspace:^"
     "@backstage/integration": "workspace:^"
     "@backstage/plugin-azure-devops-common": "workspace:^"
     "@backstage/plugin-catalog-common": "workspace:^"