diff --git a/plugins/catalog-backend-module-github/src/lib/github.test.ts b/plugins/catalog-backend-module-github/src/lib/github.test.ts
index e2303c16e6..25878c2005 100644
--- a/plugins/catalog-backend-module-github/src/lib/github.test.ts
+++ b/plugins/catalog-backend-module-github/src/lib/github.test.ts
@@ -213,6 +213,7 @@ describe('github', () => {
                 defaultBranchRef: {
                   name: 'main',
                 },
+                catalogInfoFile: null,
               },
               {
                 name: 'demo',
@@ -222,6 +223,11 @@ describe('github', () => {
                 defaultBranchRef: {
                   name: 'main',
                 },
+                catalogInfoFile: {
+                  __typename: 'Blob',
+                  id: 'acb123',
+                  text: 'some yaml',
+                },
               },
             ],
             pageInfo: {
@@ -243,6 +249,7 @@ describe('github', () => {
             defaultBranchRef: {
               name: 'main',
             },
+            catalogInfoFile: null,
           },
           {
             name: 'demo',
@@ -252,6 +259,11 @@ describe('github', () => {
             defaultBranchRef: {
               name: 'main',
             },
+            catalogInfoFile: {
+              __typename: 'Blob',
+              id: 'acb123',
+              text: 'some yaml',
+            },
           },
         ],
       };
@@ -262,9 +274,9 @@ describe('github', () => {
         ),
       );
 
-      await expect(getOrganizationRepositories(graphql, 'a')).resolves.toEqual(
-        output,
-      );
+      await expect(
+        getOrganizationRepositories(graphql, 'a', 'catalog-info.yaml'),
+      ).resolves.toEqual(output);
     });
   });
 });
diff --git a/plugins/catalog-backend-module-github/src/lib/github.ts b/plugins/catalog-backend-module-github/src/lib/github.ts
index 6a944af330..d854cc9043 100644
--- a/plugins/catalog-backend-module-github/src/lib/github.ts
+++ b/plugins/catalog-backend-module-github/src/lib/github.ts
@@ -65,6 +65,11 @@ export type Repository = {
   defaultBranchRef: {
     name: string;
   } | null;
+  catalogInfoFile: {
+    __typename: string;
+    id: string;
+    text: string;
+  } | null;
 };
 
 type RepositoryTopics = {
@@ -266,14 +271,22 @@ export async function getOrganizationTeams(
 export async function getOrganizationRepositories(
   client: typeof graphql,
   org: string,
+  catalogPath: string,
 ): Promise<{ repositories: Repository[] }> {
+  const catalogPathRef = `HEAD:${catalogPath}`;
   const query = `
-    query repositories($org: String!, $cursor: String) {
+    query repositories($org: String!, $catalogPathRef: String!, $cursor: String) {
       repositoryOwner(login: $org) {
         login
         repositories(first: 100, after: $cursor) {
           nodes {
             name
+            catalogInfoFile: object(expression: $catalogPathRef) {
+              __typename
+              ... on Blob {
+                id
+              }
+            }
             url
             isArchived
             repositoryTopics(first: 100) {
@@ -302,7 +315,7 @@ export async function getOrganizationRepositories(
     query,
     r => r.repositoryOwner?.repositories,
     x => x,
-    { org },
+    { org, catalogPathRef },
   );
 
   return { repositories };
diff --git a/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.test.ts b/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.test.ts
index 63fb77288b..8b315a8a1e 100644
--- a/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.test.ts
+++ b/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.test.ts
@@ -170,6 +170,11 @@ describe('GitHubEntityProvider', () => {
             defaultBranchRef: {
               name: 'main',
             },
+            catalogInfoFile: {
+              __typename: 'Blob',
+              id: 'abc123',
+              text: 'some yaml',
+            },
           },
         ],
       }),
@@ -267,6 +272,11 @@ describe('GitHubEntityProvider', () => {
             defaultBranchRef: {
               name: 'main',
             },
+            catalogInfoFile: {
+              __typename: 'Blob',
+              id: 'abc123',
+              text: 'some yaml',
+            },
           },
         ],
       }),
@@ -341,6 +351,11 @@ describe('GitHubEntityProvider', () => {
             defaultBranchRef: {
               name: 'main',
             },
+            catalogInfoFile: {
+              __typename: 'Blob',
+              id: 'abc123',
+              text: 'some yaml',
+            },
           },
         ],
       }),
@@ -381,6 +396,110 @@ describe('GitHubEntityProvider', () => {
       entities: expectedEntities,
     });
   });
+
+  it('should filter out invalid locations when validateLocationsExist is set to true', async () => {
+    const config = new ConfigReader({
+      catalog: {
+        providers: {
+          github: {
+            myProvider: {
+              organization: 'test-org',
+              catalogPath: 'catalog-custom.yaml',
+              filters: {
+                branch: 'main',
+              },
+              validateLocationsExist: true,
+            },
+          },
+        },
+      },
+    });
+    const schedule = new PersistingTaskRunner();
+    const entityProviderConnection: EntityProviderConnection = {
+      applyMutation: jest.fn(),
+      refresh: jest.fn(),
+    };
+
+    const provider = GitHubEntityProvider.fromConfig(config, {
+      logger,
+      schedule,
+    })[0];
+
+    const mockGetOrganizationRepositories = jest.spyOn(
+      helpers,
+      'getOrganizationRepositories',
+    );
+
+    mockGetOrganizationRepositories.mockReturnValue(
+      Promise.resolve({
+        repositories: [
+          {
+            name: 'test-repo',
+            url: 'https://github.com/test-org/test-repo',
+            repositoryTopics: {
+              nodes: [],
+            },
+            isArchived: false,
+            defaultBranchRef: {
+              name: 'main',
+            },
+            catalogInfoFile: null,
+          },
+          {
+            name: 'another-repo',
+            url: 'https://github.com/test-org/another-repo',
+            repositoryTopics: {
+              nodes: [],
+            },
+            isArchived: false,
+            defaultBranchRef: {
+              name: 'main',
+            },
+            catalogInfoFile: {
+              __typename: 'Blob',
+              id: 'abc123',
+              text: 'some yaml',
+            },
+          },
+        ],
+      }),
+    );
+
+    await provider.connect(entityProviderConnection);
+
+    const taskDef = schedule.getTasks()[0];
+    expect(taskDef.id).toEqual('github-provider:myProvider:refresh');
+    await (taskDef.fn as () => Promise<void>)();
+
+    const url = `https://github.com/test-org/another-repo/blob/main/catalog-custom.yaml`;
+    const expectedEntities = [
+      {
+        entity: {
+          apiVersion: 'backstage.io/v1alpha1',
+          kind: 'Location',
+          metadata: {
+            annotations: {
+              'backstage.io/managed-by-location': `url:${url}`,
+              'backstage.io/managed-by-origin-location': `url:${url}`,
+            },
+            name: 'generated-934f500db2ba2e8ea3524567926f45a73bb0b532',
+          },
+          spec: {
+            presence: 'optional',
+            target: `${url}`,
+            type: 'url',
+          },
+        },
+        locationKey: 'github-provider:myProvider',
+      },
+    ];
+
+    expect(entityProviderConnection.applyMutation).toHaveBeenCalledTimes(1);
+    expect(entityProviderConnection.applyMutation).toHaveBeenCalledWith({
+      type: 'full',
+      entities: expectedEntities,
+    });
+  });
 });
 
 it('apply full update on scheduled execution with topic exclusion taking priority over topic inclusion', async () => {
@@ -437,6 +556,11 @@ it('apply full update on scheduled execution with topic exclusion taking priorit
           defaultBranchRef: {
             name: 'main',
           },
+          catalogInfoFile: {
+            __typename: 'Blob',
+            id: 'abc123',
+            text: 'some yaml',
+          },
         },
         {
           name: 'test-repo-2',
@@ -455,6 +579,11 @@ it('apply full update on scheduled execution with topic exclusion taking priorit
           defaultBranchRef: {
             name: 'main',
           },
+          catalogInfoFile: {
+            __typename: 'Blob',
+            id: 'abc123',
+            text: 'some yaml',
+          },
         },
         {
           name: 'test-repo-3',
@@ -470,6 +599,11 @@ it('apply full update on scheduled execution with topic exclusion taking priorit
           defaultBranchRef: {
             name: 'main',
           },
+          catalogInfoFile: {
+            __typename: 'Blob',
+            id: 'abc123',
+            text: 'some yaml',
+          },
         },
       ],
     }),
diff --git a/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.ts b/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.ts
index 3909b5a3c2..42dd6afa5d 100644
--- a/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.ts
+++ b/plugins/catalog-backend-module-github/src/providers/GitHubEntityProvider.ts
@@ -163,6 +163,7 @@ export class GitHubEntityProvider implements EntityProvider {
   private async findCatalogFiles(): Promise<Repository[]> {
     const organization = this.config.organization;
     const host = this.integration.host;
+    const catalogPath = this.config.catalogPath;
     const orgUrl = `https://${host}/${organization}`;
 
     const { headers } = await this.githubCredentialsProvider.getCredentials({
@@ -177,8 +178,18 @@ export class GitHubEntityProvider implements EntityProvider {
     const { repositories } = await getOrganizationRepositories(
       client,
       organization,
+      catalogPath,
     );
 
+    if (this.config.validateLocationsExist) {
+      return repositories.filter(repository => {
+        return (
+          repository.catalogInfoFile?.__typename === 'Blob' &&
+          repository.catalogInfoFile.text !== ''
+        );
+      });
+    }
+
     return repositories;
   }