diff --git a/packages/e2e-test/src/commands/run.ts b/packages/e2e-test/src/commands/run.ts
index d324aeafaf..fcca2ee7f2 100644
--- a/packages/e2e-test/src/commands/run.ts
+++ b/packages/e2e-test/src/commands/run.ts
@@ -183,20 +183,23 @@ async function buildDistWorkspace(workspaceName: string, rootDir: string) {
  * Pin the yarn version in a directory to the one we're using in the Backstage repo
  */
 async function pinYarnVersion(dir: string) {
-  const yarnRc = await fs.readFile(paths.resolveOwnRoot('.yarnrc'), 'utf8');
+  const yarnRc = await fs.readFile(paths.resolveOwnRoot('.yarnrc.yml'), 'utf8');
   const yarnRcLines = yarnRc.split('\n');
-  const yarnPathLine = yarnRcLines.find(line => line.startsWith('yarn-path'));
+  const yarnPathLine = yarnRcLines.find(line => line.startsWith('yarnPath:'));
   if (!yarnPathLine) {
-    throw new Error(`Unable to find 'yarn-path' in ${yarnRc}`);
+    throw new Error(`Unable to find 'yarnPath' in ${yarnRc}`);
   }
-  const match = yarnPathLine.match(/"(.*)"/);
+  const match = yarnPathLine.match(/^yarnPath: (.*)$/);
   if (!match) {
-    throw new Error(`Invalid 'yarn-path' in ${yarnRc}`);
+    throw new Error(`Invalid 'yarnPath' in ${yarnRc}`);
   }
   const [, localYarnPath] = match;
   const yarnPath = paths.resolveOwnRoot(localYarnPath);
 
-  await fs.writeFile(resolvePath(dir, '.yarnrc'), `yarn-path "${yarnPath}"\n`);
+  await fs.writeFile(
+    resolvePath(dir, '.yarnrc.yml'),
+    `yarnPath: ${yarnPath}\n`,
+  );
 }
 
 /**